The Massachusetts Division of Fisheries and Wildlife (DFW) is engaged in an innovative, collaborative effort with the Massachusetts Highway Department (MassHighway) to understand and lessen the effects of existing and proposed major roads on populations of state-listed amphibians and reptiles. It is anticipated that this cooperative relationship will serve as a flexible and dynamic model to improve the regulatory review process and outcome for other state highway departments.  Fifteen species of amphibian and reptile are state-listed in Massachusetts as endangered, threatened, or of “special concern.” DWF and MassHighway are currently collaborating on a three-year study to determine ideal dimensions of wildlife passage structures for freshwater turtles (partially funded by the Federal Highway Administration). To complement these research objectives, the DFW proposes to broaden the focus of this study to evaluate several additional topics related to landscape connectivity for state-listed amphibians and reptiles. The DFW will investigate methods of improving the permeability of existing road and rail corridors for amphibians and reptiles, by conducting field and laboratory studies. Additionally, the DFW will develop and publicize a web-based “citizen-science” program to identify hotspots of amphibian and reptile road mortality.